Screening for anti-neoplastic enzymes producing halophilic bacterial extract and their antioxidant activity due to carotenoid synthesis

Abstract

The methanolic extracts were analyzed for anti-neoplastic enzymes screening, while antioxidant capabilities were investigated for fifteen isolates. The extracts also have enhanced radical scavenging ability against the stable ABTS (2,2’azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (86.34 ± 0.007 mM TE/g extract), nitric oxide (64.79 ± 0.004 μg/ml), phosphomolybdenum (1.69 ± 0.024 mg AAE/g extract), FRAP (ferric reducing ability of plasma) (28.19 ± 0.012 mM Fe (II) E/mg) and showed better Ic50 values on DPPH (2,2-diphenyl-2-picrylhydrazyl) (5.48 mg/ml) assay. Finally, four potent strains from various antioxidant activities were chosen. The isolates could synthesize carotenoids that possessed anti-oxidant activities was identified by UV/Visible spectra, GC–MS and LC-MS/MS analysis and confirmed that the isolated strains had the potential to produce compounds that might be used primarily as therapeutics. The enormous anti-oxidant potential of the strains Staphylococcus arlettae, Bacillus subtilis, B. tequilensis, and B. haynesii extracts could be used to prevent oxidative-stress-related illnesses like cancer, neurodegeneration, as well as in food safety markets in general.