Abstract
BackgroundCorydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC.MethodsXylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats were used to evaluate the anti-inflammatory activity of CB. The macrophage binding with high-performance liquid chromatography (HPLC) analysis and HPLC-MS were established to screen the potential active compounds. ELISA kits were performed to measure the levels of IL-6, IL-10, TNF-α and NO in RAW 264.7 macrophages culture media.ResultsThe alkaloid extract of CB could inhibit significantly xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats. Two components binded to RAW 264.7 cell were identified as 12-hydroxycorynoline and corynoline. Bioassays demonstrated that these two compounds significantly inhibited LPS-induced IL-6, IL-10, TNF-α and NO levels.ConclusionsThe results suggest that corynoline and 12-hydroxycorynoline contribute to the anti-inflammatory effects of the alkaloid extract of CB. Our findings suggest that these two compounds can be used as candidate for anti-inflammatory drugs.
Highlights
Corydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown
12-hydroxycorynoline, 6-acetonylcorynoline, corynoline and acetylcorynoline were prepared by the Nanjing University of Chinese Medicine and purity was 99 % according to high-performance liquid chromatography (HPLC) analysis
Effect of the alkaloid extract of CB on xylene-induced ear edema in mice Utilizing a typical model of inflammatory response, the anti-inflammatory activity of the alkaloid extract of CB was investigated
Summary
Corydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC. (CB; family: Corydalis) is a perennial herb containing several pharmacologically important alkaloids such as corydaline, 12-hydroxycorynoline, protopine, acetylcorynoline, and corynoline [1]. The dried whole plant is referred in traditional Chinese medicine (TCM) as Herba Corydalis Bungeanae, clearing heat and toxins, as well as anti-inflammatory [2]. Screening of bioactive compounds for use in animal models of disease is laborious and inappropriate for TCMs [6]. A screening method involving in the binding of molecules
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