Abstract
Anthrax is a deadly disease caused by Bacillus antracis that damages the lymphatic and vascular systems of animals and humans. Anthrax prevention in Indonesia has been done but has many shortcomings among them is a lot of dead animal raises after vaccination and relatively expensive. The azadirachtin bioactive compound produced by neem endophytic microbes is known to inhibit the growth of B. anthracis bacteria, thus potentially as an anti-anthrax agent. Other bacteria that have the same pathogens as B. antrachis are Methycillin Resistant Staphylococcus aureus (MRSA) so that it is used as a research object. The aim of this research is to obtain the active biomaterials from selected neem leaf endophytes to be tested in S. aureus MRSA as bacteria with Anthrax pathogens. The method used in this research is the isolation, selection, and extraction of Neem endophytes. The process of isolation is done by growing on the general media and the selection process on special media. The bioactive extraction process uses 1000 rpm centrifuge and screening on MRSA culture medium. Inhibitory zone tests were used to determine the effect of endophytic biopsy in inhibiting MRSA growth. The result of the research is that the bioactive endophytes of neem are able to inhibit MRSA growth which is characterized by the formation of drag zone around the endophytic bioactive. Thus it can be concluded that the use of neem endophytes can be a new solution to eradicate the presence of anthrax disease in the livestock and people in Indonesia with safe research methods, relatively inexpensive and ineffective vaccine replacements.
Highlights
METHODSAnthrax is an infectious disease caused by B. antrachis
The aim of this research is to obtain the active biomaterials from selected neem leaf endophytes to be tested in S. aureus Methycillin Resistant Staphylococcus aureus (MRSA) as bacteria with Anthrax pathogens
These endophytic bacteria lived within the neem leaf tissues that produced secondary metabolite compounds including azadirachtin compounds
Summary
METHODSAnthrax is an infectious disease caused by B. antrachis. Spores of this type of bacteria can survive in the extreme temperatures and chemicals. In Indonesia anthrax causes many deaths in livestock This fact proves that anthrax is so dangerous and detrimental that a new breakthrough is needed to prevent the case efficiently and effectively through neutralization mechanisms, as well as by safe research methods (Vaithiyanathan et al, 2016). Azadirachta indica leaf extract (neem) can fight bacterial pathogen Bacillus anthracis (Vaithiyanathan et al, 2016). Based on these facts, the handling of anthrax disease in livestock needs to be developed with the utilization of endophytic bacteria-based biomaterials. Microbial endophytes in neem plants have the potential to resist vegetative growth of anthrax agents
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