Abstract
In the Leonard jar method of selecting rhizobia for effectiveness, the rhizobia interact with the legume roots growing in sterile sand or vermiculite irrigated with an N-free nutrient solution. In nature, the legume roots interact with inoculant rhizobia in the soil environment in the presence of other microorganisms, native/indigenous rhizobia, and soil N. Therefore, selected strains must be tested for effectiveness under soil conditions to weed out less effective ones. In this experiment, rhizobia previously screened in Leonard jars are evaluated further in potted field soil. The effectiveness of mixed- and single-strain inocula are compared. Infective, native rhizobial populations in field soil are determined.
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