Abstract
Seven clones showing β -hemolysis, from a Listeria monocytogenes genomic library, were assessed by a hydrophobic grid-membrane filter (HGMF) screening technique for their specificity as DNA probes. Plasmid DNA from each of the clones was screened by colony hybridization against replicates of a library of 100 organisms, consisting of 70 L. monocytogenes strains, ten other Listeria spp., and 20 organisms of other genera, arrayed on an HGMF. Clones showing potential for use in analytical food microbiology were identified by this means.
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