Screening differentially expressed genes related to gastric carcinoma by gene expression profile chip

Abstract

Objective To screen genes differentially expressed in clinical samples of resected gastric carcinoma and their adjacent normal gastric tissues by gene expression profile chip.Methods Microarrays containing 14 784 individual full length cDNAs from homo sapiens were applied to screen genes differentially expressed in 7 clinical samples of resected gastric carcinoma and their adjacent normal gastric tissues.Furthermore,we have confirmed differentially expressed genes in gastric carcinoma tissue by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal gastric mucosae,35 differentially expressed genes were detected in 7 cases of gastric carcinoma tissue,including 18 genes with up-regulated expression and 17 genes with down-regulated expression.In order to verify the differentially expressed genes detected from cDNA chip experiment,7 genes with most altered expression were chosen from 7 cases of gastric carcinoma to be verified by RT-PCR,of which 5 genes were confirmed to be up-regulated (S100A6,S100A11,ETV4,CDH17 and EphB4) and 2 genes to be down-regulated (NK4 and PPP2R1B).Above all genes in gastric carcinoma were verified by RT-PCR to be in accordance with the pattern of gene microarray detection.Conclusion Microarray analysis is efficient in the screening of differentially expressed genes related to gastric carcinoma.As proved by RT-PCR on partial genes that are differentially expressed,the altered expression of S100A6,S100A11,ETV4,CDHI7,NK4,PPP2R1B and EphB4 might be involved in the oncogenesis and progression of gastric carcinoma. Key words: Gastric carcinoma; Gene chip; Differential expression