Abstract
In order to adequately plan experiments to measure tolerance of cotton to Hoplolaimus columbus, coefficients of variation (CVs) and error variances of yield were examined to determine the minimum number of replicates and environments needed to measure specified differences for tolerance index (TI) and field tolerance. In two greenhouse trials with 84 genotypes, the error variance was 1,423 and would have required 231 replicates to ensure detecting a 10% genotypic difference in TI. In the 3-yr, one-location field study reported, CVs ranged from 10.0 to 25.4%, while error variances for TI ranged from 68 to 512. The pooled error variance (243) was used in calculating the minimum number of replicates and environments [...]
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