Abstract
Clover rhizobia (55 strains) were screened for tolerance of acidity and Al, using the technique of Keyser and Munns (1979). Assessment of visible turbidity after 14 days indicated three strains tolerant of pH 4.5 (although growth rate was reduced), 25 strains tolerant of 5μ m Al and no strains tolerant of 50 μ m Al at pH 5.5.50 μ mAl caused a decrease in the numbers of acid-tolerant strains at pH 4.5. Tolerance of acidity or Al was not associated with the pH or Al status of the soil from which a strain was isolated. Screening of eight strains of clover rhizobia and nine strains of Lotus rhizobia using turbidity assessment and viable counts indicated seven strains of clover rhizobia with different degrees of tolerance of 20 μ m Al but none tolerant of 50 μ m Al at pH 5.5. All Lotus rhizobia (both slow- and fast-growers) were tolerant of 20 and 50 μ m Al at pH 5.5, with 50 μ m Al causing a reduction in growth rate. Subculturing of strains in non-stressed and stressed media had no effect on the response to 50 μ mAl at pH 5.5.
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