Screening, characterization and molecular-genetical identification of a new bacterial strain Paenibacillus species

L I Sapunova,A G Lobanok,Ya M Sysaliatsin,K K Yatsevich,I A Tamkovich,L V Yarkhova,S A Kulish

doi:10.29235/1561-8323-2019-63-2-181-188

Abstract

Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.

Highlights

Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing micro biota for further characterization
It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase, alginase, extracellular polysaccharides, and pigments, probably carotenoids
Based on cultural-morphological and physiolo gical-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene the bacterial culture was identified as Paenibacillus species PS-K-17

Summary

Introduction

Based on cultural-morphological and physiolo gical-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. M. Screening, characterization and molecular-genetical identification of a new bacterial strain Paenibacillus species. D. Collins штаммы бактерий Bacillus polymyxa на основании ряда фенотипических свойств и состава нуклеотидных последовательностей гена 16S рРНК были реклассифицированы и в качестве типовых включены

Results

Conclusion