Screening, Characterization and 16S rRNA Sequencing of Thermophilic Bacteria Producing Amylase and Protease from Pekonina Hot Springs, South Solok

Abstract

— Amylase and protease enzymes play a major role in food industries, textile, medicine, paper and detergent industries so that the level of need for amylase and protease is very high, especially those with thermophilic properties. This study aims to obtain amilase and protease-producing thermophilic bacteria, determine the growth and activity of amilase and protease enzymes and analyze the molecular-based characterization of amilase and protease-producing thermophilic bacteria from Pekonina hot springs, Solok Selatan. This research uses a survey method. The results obtained 5 bacteria isolates from the pond I and 5 bacteria isolats from pond II which can produce amylase and protease enzymes. The macroscopic observation of thermophilic bacteria showed that the colonies were circular, irregular, and rhizoid. Margins on bacterial isolates are entire, undulate, lobate, rhizoid. The elevation of the bacterial isolates were flat and convex. Colony colors obtained are white, cream and yellowish. Microscopic observation showed that all bacterial isolates were Gram-positive. The shape of the cells in the isolates were bacilli, and diplobasil and coccus. BTPAP-04 is the isolate that had the highest amylolytic index and proteolytic index values are 2.36 mm and 2.96 mm. Molecular identification of 16S rRNA was performed on BTPAP-04 isolate was identified as Geobacillus stearothermophilus strain IFO 12550 and Geobacillus stearothermophilus strain R-35646. Keywords— Thermophilic Bacteria, Amylase, Protease, Geobacillus stearothermophilus