Screening antibacterial activity ODS fractions of marine sponges against non-pathogenic bacteria tuberculosis Mycobacterium smegmatis

Deiske A Sumilat

doi:10.35800/jasm.9.1.2021.32692

Abstract

Nine unidentified marine sponges from Bunaken Island waters in Manado Indonesia were observed on anti-tuberculosis (TB) against non-pathogenic Mycobacterium smegmatis NBRC 3207 and it was found that the ethanol crude extracts and ODS fractions of these sponges inhibited the growth of bacteria M. smegmatis. Sponge SP.323 is the most inhibiting and showed Intermediate (I) activity with inhibition zones of 9, 11, 11, 8 mm/disk at 10µg/disk., for fraction 3,4,5,6 respectively, and need further experimental analysis to justify the antimicrobial activity, NMR analysis and structure elucidation.

Highlights

Tuberculosis (TB) disease is one of the top 10 causes of death and the leading cause from a single infectious agent
For several years our research has focused on antimicrobials with purpose to find new antimycobacterial substances from marine sponges and microorganisms collected in North Sulawesi, Indonesia
We found nine marine sponges collected by scuba diving at Bunaken Island in Manado Indonesia, in 2014

Summary

INTRODUCTION

Tuberculosis (TB) disease is one of the top 10 causes of death and the leading cause from a single infectious agent (above HIV/AIDS). Sumilat: Screening antibacterial activity ODS fractions of marine sponges against non-pathogenic. In the course to continue studies on anti-TB examine the inhibitory activities on the growth of C. metabolites from Indonesian marine sponges we albicans (yeast), M. hiemalis (filamentous fungus), have tested nine unidentified species of marine M. smegmatis, S. aureus (Gram-positive bacterium), sponges against M. smegmatis NBRC 3207 and E. coli (Gram-negative bacterium) (Bu et al, 2014; found that the crude extracts and ODS fractions of Abdjul et al, 2017; Maarisit et al, 2017). Metabolites from Indonesian marine sponges we albicans (yeast), M. hiemalis (filamentous fungus), have tested nine unidentified species of marine M. smegmatis, S. aureus (Gram-positive bacterium), sponges against M. smegmatis NBRC 3207 and E. coli (Gram-negative bacterium) (Bu et al, 2014; found that the crude extracts and ODS fractions of Abdjul et al, 2017; Maarisit et al, 2017) ODS column chromatography was performed to separation in 7 fractions and screening to examine the inhibitory activities on the growth of M

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION