Abstract
Objective To screen and validate the prognostic biomarkers of patients with hepatocelluar carcinoma (HCC) based on reverse phase protein array (RPPA). Methods All public RPPA data (HCC patients) in TCGA database before December 2017 were enrolled in this study. Univariate and multivariate Cox analysis were used to screen the target proteins with prognostic value. From January 2013 to January 2014, 121 HCC patients who were treated in the Affiliated Hospital of North Sichuan Medical College were enrolled in the study. Prognostic related proteins screened from RPPA data were detected in these patients’ tumor specimen. Then, the correlations between the expression of the screened protein and prognosis was further analyzed. Results In the 218 protein, 23 was related with HCC patient prognosis. Multivariate Cox regression analysis showed that high expression of NOTCH1 (HR=1.515, 95% CI: 1.287~1.845, P 0.05). Conclusion RPPA, as a high throughput protein expression quantitative technique, can be used for screening prognostic markers, and the reliability of the screening results is higher in general. Key words: Hepatocellular carcinoma; Prognosis; Biomarker; Reverse phase protein arrays
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