Abstract
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) production using new alkaliphile Microbacterium terrae KNR 9 was investigated by submerged fermentation. Statistical screening for components belonging to different categories, namely, soluble and raw starches as carbon sources, complex organic and inorganic nitrogen sources, minerals, a buffering agent, and a surfactant, has been carried out for CGTase production using Plackett-Burman factorial design. To screen out k (19), number of variables, k + 1 (20), number of experiments, were performed. Among the fourteen components screened, four components, namely, soluble starch, corn flour, yeast extract, and K2HPO4, were identified as significant with reference to their concentration effect and corresponding p value. Although soluble starch showed highest significance, comparable significance was also observed with corn flour and hence it was selected as a sole carbon source along with yeast extract and K2HPO4 for further media optimization studies. Using screened components, CGTase production was increased to 45% and 87% at shake flask level and laboratory scale fermenter, respectively, as compared to basal media.
Highlights
Starch is an important storage compound synthesized by many plants as their carbon and energy source
The aim of the present work was to screen out fourteen media components for their influence on CGTase production using a new alkaliphile isolate Microbacterium terrae KNR 9 using Plackett-Burman design
Soluble starch and raw organic carbon sources like corn flour and rice flour were selected for the study
Summary
Starch is an important storage compound synthesized by many plants as their carbon and energy source. Cyclodextrins (CDs) are produced as a result of intramolecular transglycosylation (cyclization) reaction during degradation of starch by cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) enzyme. A torus shaped microring of cyclodextrin molecule is having hydrophilic hydroxyl (-OH) groups on the outside of the ring molecule with the hydrophobic -CH groups and glycosidic oxygen located inside the cavity of the molecule. Because of this unique property, CDs can form molecular inclusion complexes with range of compounds and have found various applications [1, 3]. CDs are used in many industrial productions, analytical methods, pharmaceuticals, food and flavors, cosmetics, packing, textiles, separation processes, and so forth [3,4,5,6]. β-Cyclodextrin and its derivatives are the most widely
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