Abstract
BackgroundBiofilms can form in many industries, one of them is the food industry. The formation of biofilms in this industry could cause immense economic losses and endanger public health. Biofilms formation is mainly triggered by quorum sensing. Therefore, inhibition of quorum sensing could be an innovative approach to inhibit the formation of biofilms. One way to inhibit quorum sensing is by using anti-quorum sensing compounds. Actinomycetes are a group of bacteria that is acknowledged to produce these compounds.ResultsThere were eight crude extracts of Actinomycetes isolates that showed promising anti-quorum sensing activity against Chromobacterium violaceum. The concentration of the crude extracts was 20 mg/mL. All the crude extracts showed no antibacterial activity against food spoilage bacteria, except for crude extracts of isolate 18 PM that showed antibacterial activity against Bacillus subtilis. They also showed various antibiofilm activity, both inhibition and destruction. The highest inhibition and destruction activity sequentially was done by crude extracts of isolate 12 AC with 89.60% against Bacillus cereus and crude extracts of isolate SW03 with 93.06% against Shewanella putrefaciens.ConclusionsActinomycetes isolates that isolated from different regions in Indonesia can be used as potential candidates to overcome biofilms formed by food spoilage bacteria using their ability to produce anti-quorum sensing compounds.
Highlights
Biofilms can form in many industries, one of them is the food industry
All eight isolates showed positive anti-quorum sensing activity against indicator bacteria Chromobacterium violaceum marked by inhibition of violacein pigment (Fig. 1)
Anti-quorum sensing and antibiofilm compounds from Actinomycetes can be novel to treat biofilms, in this research are biofilms formed by food spoilage bacteria
Summary
Biofilms can form in many industries, one of them is the food industry. The formation of biofilms in this industry could cause immense economic losses and endanger public health. Biofilms formation is mainly triggered by quorum sensing. Inhibition of quorum sensing could be an innovative approach to inhibit the formation of biofilms. Bacteria can communicate with each other using chemical signaling molecules called autoinducers This communication, known as quorum sensing, is orchestrated in a cell density-dependent manner. A group of bacteria flock together that attach to a surface and enclosed in an extracellular matrix, have significant involvement in various types of industries, one of them is the food industry. The formation of biofilms in this industry can be a firm source of contamination, which responsible for causing food to spoil, which is dangerous for the safety of the food products as well as a massive economic loss. There are several bacteria such as Bacillus cereus, Bacillus subtilis, and Shewanella putrefaciens which are known for their ability to form biofilms, resulting in food spoilage
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
