Abstract
ObjectiveThe objectives of this research were to screen anti-quorum sensing activity of phyllosphere bacteria and quantify their antibiofilm activity against biofilm forming bacteria (Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium, Vibrio cholerae, Pseudomonas aeruginosa).ResultsWe found 11 phyllosphere bacteria isolates with potential anti-quorum sensing activity. Most of the crude extracts from phyllosphere bacteria isolates had anti-quorum sensing activity against Chromobacterium violaceum at certain concentration (20 and 10 mg/mL), but not crude extract from isolate JB 7F. Crude extract showed the largest turbid zone (1,27 cm) using isolate JB 14B with concentration of 10 mg/mL and the narrowest turbid zone isolate (1 cm) using JB 18B with concentration of 10 mg/mL. Crude extracts showed various antibiofilm activities against all tested pathogenic bacteria, it showed the highest biofilm inhibition (90%) and destruction activities (76%) against S. aureus.
Highlights
Nowadays, we know that about 65% of all bacterial infections were associated with bacterial biofilms [1]
We know that 1 out of 11 positive phyllosphere isolates crude extract, EJB 7B, showed antibacterial activity against all Gram positive pathogenic bacteria which used in this research
We found out that each of phyllosphere isolate has their own optimal concentrations but most of them showed activity at concentration of 20 mg/mL and all of them have no activity at concentration of 5 mg/mL Crude extract showed the largest turbid zone (1.27 cm) using isolate JB 14B with concentration of 10 mg/mL and the narrowest turbid zone isolate (1 cm) using JB 18B with concentration of 10 mg/mL (Table 1)
Summary
We know that about 65% of all bacterial infections were associated with bacterial biofilms [1]. Biofilm is an organized aggregate of microorganisms like bacteria within an extracellular polymeric matrix that they produce [1, 2]. Several pathogenic bacteria form a biofilm using a mechanism called quorum sensing. Quorum sensing is a communication form among bacteria by various types of extracellular signal molecules called autoinducer (AI). Bacteria in biofilm were more resistant to antibiotic because biofilm matrix can help with interfering the penetration of antibiotic. We need to explore compound that have capability to inhibit or destroy biofilm as well as anti-quorum sensing to control attack of biofilm-forming pathogenic bacteria [3]
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