Screening and production of bacterial amylase from different Strepomyces species

Abstract

The enzymes from the microbial sources are more stable and can be obtained from cheap sources. Amylase is most important industrial enzyme which is widely used in food and biofuel industries. Streptomyces is a group of bacteria belongs to largest genus of Actinobacteria and commonly isolated from the soil. Streptomyces species have been widely used as microbial cell in industries for production of antibiotics. For the production of antibiotics, Streptomyces species are frequently used as microbial cells. This study reported that screening and production of amylase from different Streptomyces species i.e. Streptomyces microflavus, Streptomyces cyaneus and Streptomyces diastaticus. The cultures were screened for the production of amylase by starch agar plate assay. Result shown the Streptomyces diastaticus was better amylase enzyme producer compare to Streptomyces microflavus and Streptomyces cyaneus. Streptomyces diastaticus gives maximum starch hydrolysis by showing clear zone (4.4 mm), enzyme production (1.60 U/ml) and protein concentration (2.25 mg/ml).