Abstract
In order to screen the strains with Potassium and phosphorus-releasing bacteria from the soil, the potassium-dissolving medium was used for preliminary screening, and the transparent circle method was used for re-screening. The content of soluble potassium was determined by sodium tetraphenylborate method, and the content of phosphorus was determined by anticolorimetric method of molybdenum and antimony. The strains were identified by morphological, biochemical and molecular biology. The results showed that ten strains with potassium-solubilizing function were obtained by preliminary screening of potassium solubilizing medium. The re-screening by transparent circle method showed that WP1-3 strain had the strongest potassium-solubilizing ability, with D/d value of 1.33. WP1-3 strains of decomposition of soluble potassium content is 22.918 mg/L, decompose inorganic phosphorus and organic phosphorus ability is 4.443 mg/L and 22.408 mg/L, respectively. Identifying morphological and biochemical identification showed that WP1-3 strains was gram-negative bacteria, not produce spore, the nitrogen content of WP1-3 strain increased after detection and culture for 3d and 7d were 10.825 and 38.521 mg/L. The 16SrDNA size was 1351bp, the ribosomal database comparison showed that WP1-3 strain was 99.8% similar to Rhizobium. Therefore, the WP1-3 strain belongs to the rhizobia genus.
Highlights
Potassium and phosphorus are important nutrient elements in plants and are biological nitrogen fixation must element, are the key to promote the metabolism of plant growth
Transparent circle method is used to determine the releasing bacteria screening of soluble potassium circle diameter (D/mm) and colony diameter (d/mm), calculate the circle of soluble potassium and colony diameter ratio (D/d)
The strain WP1-3 screened by the transparent circle method had the strongest potassiumhydrolyzing ability
Summary
Potassium and phosphorus are important nutrient elements in plants and are biological nitrogen fixation must element, are the key to promote the metabolism of plant growth. Most of the potassium and phosphorus in soil are found in minerals such as silicate aluminates and apatite. Potassium-hydrolyzing bacteria and phosphorushydrolyzing bacteria are a kind of bacteria isolated from soil that can decompose aluminosilicate and apatite minerals. As microbial fertilizers, they can decompose insoluble silicate aluminates such as potassium feldspar and apatite. They can decompose insoluble silicate aluminates such as potassium feldspar and apatite They can promote the transformation of insoluble nutrients such as potassium, phosphorus and magnesium into soluble nutrients, and increase the content of available nutrients in soil, promote crop growth and development, improve crop yield [2]. This paper studied the process of extraction and identification of potassium-releasing strains from soil, which provided reference for the development of new microbial fertilizer
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