Screening and optimization of a novel gallic acid and tannase production under semi quantitative and quantitative methods

Abstract

Tannin acyl hydrolase as the common name of tannase is an inducible extracellular enzyme that causes the hydrolysis of galloyl ester and depside bonds in tannins, yielding gallic acid and glucose. The main objective of this study is to find a novel gallic acid and tannase produced by Acinetobacter Baumannii. A number of optimization steps were followed in order to improve the highest production of gallic acid and tannase. In present study, A. baumannii were isolated from ICU burn. A. baumannii was examined by microscopic examination, morphological and biochemical assay including oxidase and catalase. The following parameters were considered and determined the effect of pH, temperature, incubation period and inoculum volume on gallic acid and tannase production. It was observed that A. baumannii gave the highest yields of gallic acid and tannase using semi-quantitative and quantitative methods. A novel A. baumannii stimulated production of gallic acid and tannase using 0.5% tannic acid in agar medium. The maximum production of gallic acid and tannase by A. baumannii was recorded at pH 5.5, temperature at 37°C, 72h of incubation period and 10% of the inoculum volume. The results concluded that the highest yields of gallic acid (47.54 mg/ml) and tannase (50.12U/mg) were obtained under improved conditions.