Abstract
Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts of pectinase and possess wide ranges of stability factors like temperature and pH. In this study, 17 out of 29 bacteria (58.62%) from forest soil samples were pectinolytic. However, only four bacteria (S-5, S-10, S-14, and S-17) showed high pectin hydrolysis zones (ranging from 0.2 cm to 1.7 cm). These four bacteria were identified based on colony morphology, microscopic characterization, biochemical characteristics, and 16S rDNA sequencing. They were designated as Streptomyces sp. (S-5, S-14), Cellulomonas sp. (S-10), and Bacillus sp. (S-17). Interestingly, bacteria showed cellulase and xylanase activity in addition to pectinase. The quantitative assay for pectinase activity of the four isolates provided proof that they are pectinase producers and can be considered potential candidates for industrial uses. The crude enzyme extracts of these bacteria are applicable in oil and juice extraction from sesame seeds and apples, respectively.
Highlights
Pectinases catalyze the degradation of pectic polysaccharides into simpler molecules like galacturonic acids [1]
Pectinases are exploited for bleaching pulp and recycling wastepaper, animal feed, vegetable oil extraction, and pretreatment of wastewater produced from different fruit juice industries
Novel pectinase-producing bacteria were isolated from boreal forest soil in Thunder
Summary
Pectinases catalyze the degradation of pectic polysaccharides into simpler molecules like galacturonic acids [1]. Pectinases are found in bacteria, fungi, yeasts, plants, and insects [2]. The biotechnological potentials of pectinase from microorganisms are of great interest due to their broad substrate specificity and versatility [1]. Pectinases are widely used in industrial applications such as processing fruits and vegetables, production and clarification of juice, fermentation of tea and coffee. Pectinases are exploited for bleaching pulp and recycling wastepaper, animal feed, vegetable oil extraction, and pretreatment of wastewater produced from different fruit juice industries. Pectinases are applied for bioethanol production, liquefaction and saccharification of biomass, bio-scouring of cotton fiber, retting and degumming plant fiber, and oil extraction [3,4,5]
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