Abstract
Biosurfactant are surface active compounds (SAC), amphiphilic in nature consisting of hydrophobic (long chain fatty acids) and hydrophilic domain (amino acid, protein, carbohydrate). Classification of this compound is based on molecular weight, mode of action and physico-chemical structure. Biosurfactant are being utilized in laundry formulation, house hold cleaning products, as antimicrobial agents, in detergents, pesticides, food and pharmaceutical industries. Various type of microorganism has been studied capable of synthesizing wide range biosurfactant by utilizing hydrocarbon compounds as source of carbon and energy. Present study aims to isolate biosurfactant producing bacteria from different waste sources. In this study among twenty-one isolated microbial cultures from different waste samples were screened for biosurfactant production using methods like hemolytic activity, blue gar assay, hydrocarbon tolerance assay, oil spread test, Emulsification index (E24%).
Highlights
Hydrocarbon compounds are widely used in vehicles are among the potential contaminant
In this study among twenty-one isolated microbial cultures from different waste samples were screened for biosurfactant production using methods like hemolytic activity, blue gar assay, hydrocarbon tolerance assay, oil spread test, Emulsification index (E24%)
The results of each sample after 6 days incubation which were analyzed for production and characterization of biosurfactant are discussed here
Summary
Hydrocarbon compounds are widely used in vehicles are among the potential contaminant. Release of this contaminants to the environment and petroleum derived compound are major cause of contamination. Most of surfactant are synthesized chemically, and are partially biodegradable, causing noxious effect on environment and human health. Microbial surfactant is more effective and versatile than many synthetic chemical surfactant owing their potential action, are biodegradable, and stable at high temperature. The structure of biosurfactant includes mycolic acid, glycolipid, polysaccharide lipid complex, lipoprotein, lipopeptides, phospholipids and microbial cell surface itself.[1] The application of biosurfactant ranges from agriculture to cosmetics; from food to petrochemical and from textiles to petroleum production,[2,3] oil recovery; remediation of organic and metal contaminated sites.[4]
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