Abstract
Objective To screen human midkine (h-midkine) inhibitory peptides from the phage display peptide library.Methods Recombinant human midkine (rh-midkine) protein was used to search for the specific peptides by panning.The specific binding activities of positive clones to target protein were examined by phage enzyme linked immunosorbent assay (ELISA).The effects of binding peptides on HepG2 cells proliferation were analyzed by methyl thiazol tetrazolium (MTT) method.Results Thenty positive clones were obtained after three rounds of selection.Three linear peptides and three cycle peptides,more frequently occurring in the affinity-selected phage population and higher specificity to target protein,were chemically synthesized.The synthetic peptides produced inhibition of the HepG2 cells proliferation to different degrees,the MP-3 (CTSTAMDNC) has the strongest inhibitory effect for the HepG2 proliferation (P ＜ 0.01),the inhibition rate is about 40.7％.Conclusion Four peptides that can specifically bind to MK protein have been screened successfully,and these four peptides show suppression effects on the proliferation in tumor cells. Key words: Midkine ; Phage display ; Peptide
Published Version
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