Abstract

Muscle tissue is involved with every stage of life activities and has roles in biological processes. For example, the blood circulation system needs the heart muscle to transport blood to all parts, and the movement cannot be separated from the participation of skeletal muscle. However, the process of muscle development and the regulatory mechanisms of muscle development are not clear at present. In this study, we used bioinformatics techniques to identify differentially expressed genes specifically expressed in multiple muscle tissues of mice as potential candidate genes for studying the regulatory mechanisms of muscle development. Mouse tissue microarray data from 18 tissue samples was selected from the GEO database for analysis. Muscle tissue as the treatment group, and the other 17 tissues as the control group. Genes expressed in the muscle tissue were different to those in the other 17 tissues and identified 272 differential genes with highly specific expression in muscle tissue, including 260 up-regulated genes and 12 down regulated genes. is the genes were associated with the myofibril, contractile fibers, and sarcomere, cytoskeletal protein binding, and actin binding. KEGG pathway analysis showed that the differentially expressed genes in muscle tissue were mainly concentrated in pathways for AMPK signaling, cGMP PKG signaling calcium signaling, glycolysis, and, arginine and proline metabolism. A PPI protein interaction network was constructed for the selected differential genes, and the MCODE module used for modular analysis. Five modules with Score > 3.0 are selected. Then the Cytoscape software was used to analyze the tissue specificity of differential genes, and the genes with high degree scores collected, and some common genes selected for quantitative PCR verification. The conclusion is that we have screened the differentially expressed gene set specific to mouse muscle to provide potential candidate genes for the study of the important mechanisms of muscle development.

Highlights

  • Muscles represent a crucial group of soft tissues derived from the mesoderm that are primarily responsible for locomotion, and movement in all animals butthe World Health Organization estimates musculoskeletal disorders cause the highest proportion of disabilities worldwide, affecting approximately 1.7 billion people [1]

  • Gene expression in muscle to the expression profiles of all 17 control tissues combined, allowed for the identification of 260 DEGs that were upregulated, and 12 differentially expressed genes (DEGs) that were downregulated in muscle

  • Myosin heavy chain 1 (Myh1) was found to have the highest log2 of 6.675, which is indicative of a more than 100-fold greater expression in muscles relative to the combination of the 17 control tissues used in this study

Read more

Summary

Introduction

Muscles represent a crucial group of soft tissues derived from the mesoderm that are primarily responsible for locomotion, and movement in all animals butthe World Health Organization estimates musculoskeletal disorders cause the highest proportion of disabilities worldwide, affecting approximately 1.7 billion people [1]. There are three major group of muscles i.e., skeletal, myocardium and smooth muscles. Skeletal muscles weigh about 40% of adult weight in humans, and represent the main subgroup of muscles that allow for locomotion in conjunction with the skeletal system. Apart from locomotion, skeletal muscles have other important functions e.g., heat production, support and protection of other soft tissues, and participation in metabolic homeostasis [2, 3]. Diseases affecting skeletal muscles can result in wide ranging pathologies, and represent a key cause of morbidity and disability in human populations

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call