Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent

Abstract

Lignin is the major polluting and colouring constituent present in pulp and paper mill effluent. To degrade lignin and its derivatives, bacterial enzymes can play an important role due to stability at extreme environmental conditions. This study explored the degradation of pulp and paper mill effluent by a rod-shaped Gram-positive bacterial strain RJH-1, isolated from sludge, based on its efficiency to reduce COD, colour, AOX and lignin content. This bacterial isolate was able to grow in nitrogen-free Jensen medium. Further, RJH-1 was identified as Brevibacillus agri strain after 16 s rRNA gene sequencing. Degradation potential of this isolated bacterial strain was evaluated by batch and semi-continuous reactor study. In batch study, the isolate reduced 69 % COD, 47 % colour, 37 % lignin and 39 % AOX after 5 days whereas in control flask, 40 % COD, 26 % colour, 19 % lignin and 22 % AOX reduction was observed by the indigenous bacteria present in wastewater. During semi-continuous reactor study, it reduced 62 % COD, 37 % colour, 30 % lignin and 40 % AOX of effluent at a retention time of only 32 h whereas the reduction in control reactor was 36 % COD, 21 % colour, 18 % lignin and 29 % AOX. This study confirmed that the B. agri has the potential to degrade the lignin and reduce the colour and COD of the pulp and paper mill waste water.