Abstract
Rapid and efficient methods for the determination of cured tuberculosis (TB) are lacking. A total of 85 differentially expressed serum proteins were identified by iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) analysis (fold change >1.50 or <0.60, P < 0.05). We validated albumin (ALB), Rho GDP-dissociation inhibitor 2 (ARHGDIB), complement 3 (C3), ficolin-2 (FCN2), and apolipoprotein (a) (LPA) using the enzyme-linked immunosorbent assay (ELISA) method. Significantly increased ALB and LPA levels (P = 0.036 and P = 0.012, respectively) and significantly reduced ARHGDIB, C3, and FCN2 levels (P < 0.001, P = 0.035, and P = 0.018, respectively) were observed in cured TB patients compared with untreated TB patients. In addition, changes in ALB and FCN2 levels occurred after 2 months of treatment (P < 0.001 and P = 0.030, respectively). We established a cured TB model with 87.10% sensitivity, 79.49% specificity, and an area under the curve (AUC) of 0.876. The results indicated that ALB, ARHGDIB, C3, FCN2, and LPA levels might serve as potential biomarkers for cured TB. Our study provides experimental data for establishing objective indicators of cured TB and also proposes potential markers for evaluating the efficacy of anti-TB drugs.
Highlights
Bacteria[5], and sputum cultures require 4 to 8 weeks of incubation time[6]
We identified 85 differentially expressed proteins by iTRAQ labeling coupled with 2D-LC MS/MS in cured patients and untreated smear-positive TB patients
Most of the differentially expressed proteins were involved in the metabolic process (30, 5.57%), cellular component organization or biogenesis (28, 5.19%), and response to chemical stimulus (28, 5.19%)
Summary
Nahid et al.[9] used the SOMAscan proteomics method and found differential changes in serum extracellular matrix protein 1, the tyrosine-protein kinase Yes, insulin-like growth factor-binding protein 1, cathepsin Z, coagulation factor V and serum amyloid A proteins between TB patients with 2 months treatment and untreated TB patients. Differential serum proteins were screened using iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LCMS/MS) to identify potential biomarkers for cured TB. The candidate protein biomarkers were validated as predictors of anti-TB treatment outcomes. Our study provides experimental data for establishing an objective indicator of cured TB. It provides potential markers for evaluating the efficacy of anti-TB drugs
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