Abstract
The leaves of Morus alba (LMA) are crucial traditional Chinese medicine (TCM) of clearing heat. In ancient Chinese materia medica and the current Pharmacopoeia of the People's Republic of China, LMA are recorded to be harvested after frost for medicinal purpose. However, the reason and mechanism of this traditional usage have been still unknown so far. In this work, it was confirmed firstly that the antipyretic effect of LMA after frost was better than that of before frost significantly on feverish rats. Subsequently, the chemical profiles of LMA before and after frost were characterized by fingerprint, respectively. Then, the endemic peaks after frost and positive differential peaks were screened as the research object of spectrum-effect correlation by orthogonal signal correction partial least square discrimination (OPLS). Finally, a multivariable and continuous-index spectrum-effect correlation model coupled with OPLS was established. As a result, the antipyretic components of postfrost LMA were screened and identified as citric acid derivative and tryptophan which may be the synergistic material basis. The study can provide a scientific foundation for the enhancement of effects in the postfrost LMA. Moreover, the strategy of this research could provide a valuable reference for revealing the material basis of synergetic or antagonistic effects among other complex drug systems.
Highlights
Morus alba L. is an essential economic crop worldwide and a rapid-growing woody plant
Taking time as independent variables, ΔT (T− T6,5 h) as dependent variables, draw a temperature curve, which is shown in Figure 1. e temperature fluctuations of control group (CG) changed slightly. e rectal temperatures of model group (MG) were higher than the temperatures of other groups from 8.5 h to 18.5 h, indicating that the yeastinduced feverish model was successful
After drug administration at 6.5 h, the rectal temperatures of MG, acetaminophen group (AG), prefrost administrated groups, and postfrost administrated groups increased firstly, reached the highest at the time-point of 8.5 h, and decreased (Table 2). e body temperatures from 7.5 h to 16.5 h were significantly different between post-AG and MG (p < 0.01), while there were no significant differences between pre-AG and MG
Summary
Morus alba L. is an essential economic crop worldwide and a rapid-growing woody plant. LMA can be used for making tea and noodles. It is a kind of popular TCM, often used for dispelling wind, clearing heat, clearing lung, and moisturizing dryness [1]. It contains abundant secondary metabolites, such as polyphenols, flavonoids, and alkaloids [2], which exhibit many bioactivities, such as hypoglycemic, antioxidant, anti-inflammatory, and antipyretic activity [3]. There have only been a small number of reports on the antipyretic material of LMA; it is necessary to further study the antipyretic material bases to provide scientific evidence for quality control and safe usage
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