Abstract
Using sludge at the bottom of Hengshui Lake as samples, and screened out the SJ-9 strain with PHA synthesis capability by enrichment, purification, Nile blue staining, far-infrared spectroscopy and gas qualitative and quantitative analysis. The infrared scanning of SJ-9 bacteria product showed that this product was middle chain PHA. Optimization of SJ-9 fermentation conditions obtained the optimal conditions with optimal carbon source of sodium butyrate, optimal C/N of 35, optimal fermentation time of 120 h, and optimal initial pH for culture substrate of 7.0. 10 L fermenter amplification culture under these conditions showed consistent results with flask culture, and the bacteria and PHA had the highest yields at 120 h, with the maximal yields of 6.81 and 2.36 g/L, respectively.
Highlights
Facing the constantly increasing threat of oil resource depletion and environmental pollution in the past two decades, PHA has attracted more and more attention because it can be completely biodegraded and can be used to synthesize renewable resources [1]
Optimization of SJ-9 fermentation conditions obtained the optimal conditions with optimal carbon source of sodium butyrate, optimal C/N of 35, optimal fermentation time of 120 h, and optimal initial pH for culture substrate of 7.0
1) Screening of PHA producing strains Under UV lamp, fluorescent colonies were picked from Nile blue plate and stained with Sudan black, five strains were screened with PHA synthesis capability
Summary
Facing the constantly increasing threat of oil resource depletion and environmental pollution in the past two decades, PHA (poly-hydroxy fatty acid esters) has attracted more and more attention because it can be completely biodegraded and can be used to synthesize renewable resources [1]. With more and more in-depth understanding of PHA, its application has been developed. Biological synthesis of PHA has low yield and high cost. How to improve PHA yield and reduce PHA production cost are still key for its development and application. This study aimed to use activated sludge screening to obtain strains with high PHA yield and study the fermentation characteristics of PHAproducing strains to increase PHA yield, reduce PHA production cost, and to further expand the development and application of PHA and benefit mankind
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