Abstract
AIM:To explore molecular mechanism of gastric carcinogenesis, we screened associated genes of gastric dysplasia and further investigated their expression in gastric carcinomas with different stages. METHODS:Relatively pure dysplasia and normal tissue were procured by manual microdissection, amplified by cDNAPCR, and then used to carry out forward (dysplasia as tester, normal tissue as driver) and reverse (normal tissue as tester, dysplasia as driver) SSH. Subtracted cDNA fragments were cloned into vector, screened, sequenced, and made homologous analysis. The expression of differentially expressed fragments was detected and verified by Dot hybridization and reverse transcription-PCR. RESULTS:Two subtracted cDNA libraries were constructed. Twenty-one of 26 sequenced clones were verified to be expressed differentially. It was noted that differentiall expressions of 4 genes (P125,cytochrome c oxidase subunit I, meprin A,acidic calponin)were detected simultaneously in dysplasia, early cancer and advanced cancer.
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