Abstract
Two highly efficient simultaneous heterotrophic nitrification and aerobic denitrification (SND) bacteria, isolated from activated sludge samples which domesticated in laboratory, were identified by 16S rDNA as Pseudomonas sp. QU02 and Paracoccus sp. QU07. The factors influencing the denitrification of Pseudomonas sp. QU02 and Paracoccus sp. QU07. SND were investigated. For Pseudomonas sp. QU02, the optimum carbon source was sodium succinate, the optimum C/N was 10, the optimum initial pH was 8, and the optimum NaCl concentration was 5 g/L. The optimum carbon source for denitrification of Paracoccus sp. QU07 was sodium succinate, the optimum C/N was 10, and the optimum initial pH was 7, the optimum NaCl concentration was found to be 5 g/L, and salt tolerance response in two strains. The SND process was characterized under optimized conditions. The N removal rate of two strains were higher than 90% after 72 h SND. The enhanced denitrification of industrial wastewater by mixed SND strains was investigated. After 144 h SND, N removal rate is obviously better than unstrengthened. The mixed SND strains have potential practical application value in enhancing SND of industrial wastewater.
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