Screening and comparative studies of conducting polymers for developing effective molecular imprinted sensors for copper, zinc superoxide dismutase

Abstract

This work describes the design, development, and screening of conducting polymers based molecularly imprinting sensors (MIP) for copper, zinc superoxide dismutase (SOD1). It is clinically significant for a wide variety of cardiovascular, neurodegenerative, Covid-19, and chronic immune illness. The SOD1 MIP sensors were undertaken by electropolymerization of various monomers on Screen Printed carbon electrode (SPCE) using cyclic voltammetry (CV) to examine the molecular recognition capability. The MIP receptors film binding towards SOD1 was studied by fitting experimental CV data to the Langmuir and Freundlich isotherms. Among the various monomers EDOT (3,4-ethylenedioxythiophene), Py (Pyrrole), and DA (Dopamine), the binding affinity (KL) of the poly(3-amionphenylboronic acid) (P3APBA) imprinted MIP system was considerably higher than the other conducting polymer MIP systems. Based on the above studies, 3APBA was chosen to develop a molecularly imprinted poly(3-aminophenylboronic acid) (MIP3APBA) sensor for sensitive and selective detection of SOD1. This MIP3APBA sensor’s behavior and analytical ability were characterized by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS). It showed a lowest detection limit of 0.4 μM and a linear range of 1 μM to 500 μM. Further, this electrochemical MIP3APBA sensor was also used to quantify SOD1 levels in plasma samples.