Abstract
Sex-specific markers are critical for understanding sex determination mechanism and development of unisexual breeding in fish. Spinibarbus hollandi is an important commercial aquaculture species in southeastern China. Here, through whole genome sequencing of three female and six male individuals of S. hollandi, we first screened out a total of 88 candidate male-specific sequences. After PCR validation, three male-specific markers were proved to be able to realize male-specific amplification in S. hollandi. Subsequently, the three markers were also examined in another 24 female and 24 male individuals of S. hollandi, and the accuracy was 100%. Moreover, several male-specific genes that participate in sex determination process were identified by NR annotation, which might act as sex determining genes in S. hollandi. In general, the male-specific markers suggested an XY sex determination system in S. hollandi and would contribute a lot to understanding of sex determination mechanisms and development of unisexual breeding.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
