Abstract
ObjectiveThis research was aimed to isolate cellulolytic molds in empty fruit bunches of oil palm (EFBOP) and soils from palm oil plantation area and identify their enzyme activities to digest EFBOP.ResultsA total of seven molds were successfully isolated and screened for their enzyme activities from EFBOP and the soils. The enzymes from each isolate were produced in submerged culture using Mineral Mandels and 3% of alkali pretreated pollard in triplicates. The results indicated that all of the isolates were able to hydrolyze Carboxymethyl Cellulose (CMC), Whatmann No. 1 filter paper, and also EFBOP to sugars with reducing ends that reacted to 3,5-Dinitrosalicylic acid (DNS). The CMCase activity of isolate X showed the highest while the lowest was found for isolate MT8. Filter paperase (FPase) activity of isolate X performed the highest wile the lowest were found from isolate MT3 and MT6. The saccharification activity of isolate P showed the highest while MT6 performed the lowest activity
Highlights
Empty fruit bunches of oil palm (EFBOP) is one of the solid wastes produced from CPO mills which have not been utilized commercially
Regarding to the Filter paperase (FPase) and saccharification activity, isolate T, P, and X displayed the highest activity while isolate MT6 showed the lowest activity
Cellulolytic molds were successfully isolated from EFBOP and soil in palm oil plantation area
Summary
Empty fruit bunches of oil palm (EFBOP) is one of the solid wastes produced from CPO (crude palm oil) mills which have not been utilized commercially. Its use is only limited as fuel at the CPO mill itself or uses as a compost in oil palm plantations. These wastes are potentially to be recycled and to produce synthetic biofuel for power generation, bioethanol, as well as biocomposite product from the cellulose and lignin [1]. EFBOP is a substrate for the growth of variant molds, such as Trichoderma, Aspergillus, Mucor, Penicillium, and Neurospora crassa [2, 3]. These cellulolytic molds can hydrolyze cellulose fibrous materials into oligosaccharides, cellobiose, and glucose.
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