Abstract
AbstractJute (Corchorus capsularis L.) is an annual herb of the bast fiber plant and has great potentials in food and medicinal usages because of its various bioactivities. In this study, ultrafiltration coupled with high-performance liquid chromatography-mass spectrometry was established for screening xanthine oxidase inhibitors from the jute leaves extract. Under the optimum screening conditions, three inhibitors were successfully screened and identified as chlorogenic acid, echinacoside, and isorhamnetin-rutinoside with UV and MS data. The fluorescent quenching analysis showed that three inhibitors quenched the fluorescence intensities of enzyme with different binding capacities. For further exploring the bioactivity of three inhibitors, the protective effects on hydrogen peroxide-induced oxidative stress was investigated using human normal liver cell (LO2), human gastric mucosal epithelial cell (GES-1), and human umbilical vein endothelial cell (HUVEC). As a result, they exhibited protective effects on three injured cells in dose-dependent manners without cytotoxicity. To evaluate the difference among different jute species obtained in our laboratories, the amounts of three compounds in ten samples were assessed and analyzed. The results showed that it could be divided into three groups. The jute leaves showed nutrient and medical potentials and deserved further research on pharmaceutical and biochemical utilization in future.
Highlights
Jute (Corchorus capsularis L.) is an annual herb of the bast fiber plant that belongs to the family of Tiliaceae
Freeze-dried powder of xanthine oxidase, chlorogenic acid, and superoxide dismutase kit were purchased from Yuanye Biotechnology Co. (Shanghai, China)
500 μL of the mixture was transferred to the ultrafiltration centrifuge tube (YM-30, 10 kDa, Microcon, Merck Millipore, Germany), and the tube was centrifuged at 12,000 rpm for 20 min under 4°C (Beckman Coulter Allegra 64R, Brea, CA, USA)
Summary
Jute (Corchorus capsularis L.) is an annual herb of the bast fiber plant that belongs to the family of Tiliaceae. Secondary metabolites from plants attracted widespread attention because of their abundant activities and benefits for human health Among these secondary metabolites, phenolic compounds were well explored and studied. The control of uric acid by inhibiting the activity of xanthine oxidase is required in curing gout and related diseases [7]. UFLC-MS showed many advantages such as simple procedures, speed analysis, and low cost for the whole experiment, and it could accelerate the efficiency of active compounds discovery [17] Due to these advantages, UF-LCMS was widely applied in screening of many types of enzyme inhibitors [18,19,20,21]. Xanthine oxidase inhibitors in jute leaves extract were screened and identified by UF-LCMS. This research could give more evidence on the application of jute leaves in food and nutrients preventing some chronic diseases
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