Abstract
In order to study the changes of plant resistant genes (PRGs) in the secondary growth stems of chlorophyll deficient mutant (CDM) of A. heterophyllus , 9 630 PRGs were selected from the transcriptome data. After removing the low abundance (FPKM≤0.5), the differentially expressed genes (DEGs) were screened, and 2 513 DEGs were subjected to weighted gene coexpression network analysis (WGCNA). There are 183 DEGs in the module with high type correlation. The module genes were analyzed for GO function annotation, classification, transcription factors (TFs) and receptor-like kinases (RLKs). The results showed that module genes were mainly annotated in functional groups such as binding, catalytic activity, and metabolic process. TFs analysis showed that there are 7 families, 14 transcription factors. RLKs analysis found that 11 of the 12 DEGs in the RLK-Pelle_DLSV subfamily were up-regulated in CDM, indicating that the subfamily plays an important role in resisting stress of secondary growth stems of A. heterophyllus CDM. This study used transcriptome sequencing data to analyze the changes of PRGs in the secondary growth stems of A. heterophyllus CDM, providing data for the study of photosynthesis in response to stress of woody plant stems.
Highlights
Secondary growth of woody plant stems is related to the formation and quality of wood, so it has important economic and scientific research value to study it (Wang and Lu, 2009)
In order to study the changes of plant resistant genes (PRGs) in the secondary growth stems of chlorophyll deficient mutant (CDM) of A. heterophyllus, 9 630 PRGs were selected from the transcriptome data
The 2 513 selected differentially expressed genes (DEGs) were divided into four parts: I (Unique gene in CK), II (Common genes of CK and CDM are down-regulated in CDM), III (Common genes of CK and CDM are up-regulated in CDM) and IV (Unique gene in CDM)
Summary
Secondary growth of woody plant stems is related to the formation and quality of wood, so it has important economic and scientific research value to study it (Wang and Lu, 2009). Chlorophyll content in many plants is related to various disease resistance (Ou, 2007, Anhui Agricultural Science Bulletin, 13(6): 134-135). Plant defense response is mainly generated through the expression of plant resistant gene (PRGs) (Liu et al, 2008, Anhui Agricultural Sciences, 36(13): 5342-5343, 5417). Fu et al (2018, Journal of Tropical Crops, 39(6): 1081-1086) studied the characters of chlorophyll deficient mutant (CDM) of Artocarpus heterophyllus and found that its overall growth rate was worse than that of normal plants, which may be related to the low content of chlorophyll in vivo and the inability to carry out normal photosynthesis. Transcriptome sequencing data were used to analyze the changes of PRGs in the secondary growth stems of Artocarpus heterophyllus CDM, providing data for the study of photosynthesis in response to stress of woody plant stems
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