Abstract
Summary Baihe Zhimu Tang (BZT) is a widely used traditional Chinese medicinal formula for the treatment of various diseases; however, its active components have remained unknown. In this study, high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) methods were developed for the analysis of metabolic components of BZT. The HPLC experiments used a reversed-phase Shim-Pack VP-ODS C18 column with the column temperature at 35°C and a mobile phase system consisting of aqueous formic acid (0.05%, υ/υ) and acetonitrile using a gradient elution at the flow rate of 0.2 mL/min−1. The ESI-MS was operated with a single-quadrupole mass spectrometer in both negative and positive ion modes. After oral administration of extraction of BZT, the rat's plasma, urine, and feces were also analyzed; 37 metabolic components including 23 original components and 14 transformative components of BZT were detected. The analysis of metabolic components in BZT by HPLC-ESI-MS methods could be a us...
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