Screening alcohol degrading function probiotics and protective effect of fermented apple juice on alcoholic liver injury in mice

Abstract

In this study, we screened probiotics with alcohol degrading function and studied the antioxidant activity of fermented apple juice and its protective effect on alcoholic liver injury in mice. Lactobacillus helveticus CICC6064 was screened by the test of alcohol degradation and simulated gastrointestinal digestion in vitro. The antioxidant activity, polyphenol and flavonol concentrations of fermented apple juice were increased. Animal experiments showed that compared with other experimental groups, Lactobacillus helveticus CICC6064 fermented apple juice could reduce the concentration of alcohol in serum of mice significantly, meanwhile, it also can protect the liver by reducing the pro-inflammatory cytokines (TNF-α, IL-1β, IL-2, and IL-6), increasing anti-inflammatory cytokines (IL-10), reducing oxidative stress in the body (increasing SOD, GST, CAT, reducing NO), reducing liver toxicity index (ALT, AST, GGT, ALP), and reducing liver lipid accumulation.