Abstract
Multiclass screening of drugs with high resolution mass spectrometry is of great interest due to its high time-efficiency and excellent accuracy. A high-scale, fast screening method for pesticides in fishery drugs was established based on ultrahigh performance liquid chromatography tandem quadrupole-Orbitrap high-resolution mass spectrometer. The target compounds - were diluted in methanol and extracted by ultrasonic treatment, and the extracts were diluted with MeOH-water (1:1, v/v) and centrifuged to remove impurities. The chromatographic separation was performed on an Accucore aQ-MS column (100 mm × 2.1 mm, 2.6 μm) with gradient elution using 0.1% formic acid in water (containing 5 mmol/L ammonium formate) and 0.1% formic acid in methanol (containing 5 mmol/L ammonium formate) in Full Scan/dd-MS2 (TopN) scan mode. A screening database, including mass spectrometric and chromatographic information, was established for identification of compounds. The screening detection limits of methods ranged between 1–500 mg/kg, the recoveries of real samples spiked with the concentration of 10 mg/kg and 100 mg/kg standard mixture ranged from 70% to 110% for more than sixty compounds, and the relative standard deviations (RSDs) were less than 20%. The application of this method showed that target pesticides were screened out in 10 samples out of 21 practical samples, in which the banned pesticide chlorpyrifos were detected in 3 out of the 10 samples.
Highlights
Aquaculture is estimated to provide half of aquatic products by 2030 from the farming of freshwater or marine areas [1]
Our study aims to develop a more generic screening method for a wider scope of pesticides with a self-built database, which can keep the advantages of robustness, simplicity, and time-efficiency
The data was acquired after parameter optimization on ultrahigh performance liquid chromatography interfaced quadrupole-Orbitrap mass spectrometer
Summary
Aquaculture is estimated to provide half of aquatic products by 2030 from the farming of freshwater or marine areas [1]. According to the “Green food—fishery medicine application guideline (NY/T 755-2013)” in the Agricultural Industry Standards of the People’s Republic of China [2], fishery medicine refers to the substances that prevent or treat diseases in aquaculture animals or purposefully regulate the physiology of animals, including chemicals, antibiotics, Chinese herbal medicines and biological products. It is known as chemical inputs or veterinary medicinal products (VMPs) applied in aquaculture in Europe and the United States [3,4]. Chemical inputs from aquaculture include antifoulants, antibiotics, parasiticides, anesthetics and disinfectants [5], while parasiticides in fishery mainly contain avermectins, pyrethroids, hydrogen peroxide, and organophosphates [5,6].
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