Screen and preliminary function study of fetal liver specific oncofetal RNA AK003710

Abstract

Objective To screen for a fetal liver specific oncofetal RNA and to study its effect on the proliferation and mobility of hepatocellular carcinoma(HCC)cells.Methods By overlapping the microarray results of mouse fetal liver and regenerated liver following partial hepatectomy(PH),we obtained several candidate lncRNAs which were highly expressed in both fetal liver and regenerated liver tissues,and then the expression of these candidate lncRNAs in HCC tissue were also detected.The most differentially expressed lncRNA in the mouse HCC tissues as detected by real-time PCR were chosen for further research.By real-time PCR,the expression of those lncRNA in fetal liver and regenerated liver tissue was verified.For the function study,EdU labeling system and Transwell experiment were carried out to determine the proliferation ability and mobility after knocking down the lncRNA by siRNA transfection in HCC cell line Hepa1-6.Results Seven candidate lncRNAs which were highly expressed in both fetal liver and regenerated liver tissues were obtained;3of them were overexpressed in HCC tissues compared with normal liver tissues,3had no differential expression,and 1 was undetectable.Among the 3overexpressed lncRNAs,lncRNA-AK003710 was the most differentially expressed lncRNA according to microarray and realtime PCR results.And then we detected that lncRNA-AK003710 was overexpressed in the fetal liver and 3kinds of injured liver tissues.Furthermore,the proliferation ability and mobility of Hepa1-6were impaired after knocking down lncRNA-AK003710 in HCC cell line Hepa1-6.Conclusion lncRNA-AK003710 is overexpressed in the fetal liver,regenerated liver tissues,and HCC tissues;therefore it is an oncofetal RNA that can regulate the proliferation and invasion of HCC cells and may be a potential therapeutic target for HCC.