Abstract
Microglia activation and the release of various inflammatory cytokines are largely related to neurological diseases, including Parkinson’s, Alzheimer’s, and other brain diseases. The suppression of microglial cells using natural bioactive compounds has become increasingly important for brain therapy owing to the expected beneficial effect of lower toxicity. Scoparone (6,7-dimethoxycoumarin), a major bioactive compound found in various plant parts, including the inner shell of chestnut (Castanea crenata), was evaluated on lipopolysaccharide (LPS)-activated BV-2 microglia cells. The results indicated that scoparone suppresses the LPS-stimulated increase of neuroinflammatory responses and inhibited the pro-inflammatory cytokine production in the BV-2 microglial cells. A mechanistic study showed that scoparone specifically inhibited the LPS-stimulated activation via a major regulation of IRF-3 and a regulation of ERK, whereby the phosphorylation in the BV-2 microglial cells is blocked. These data suggest that scoparone has anti-neuroinflammatory effects in LPS-activated BV-2 microglial cells, and could possibly be used in the development of novel drugs for the prevention and treatment of neuroinflammatory diseases.
Highlights
Neurons and microglial cells are the integral part of the central nervous system, in which the microglial cells occupy 5%–10% of the brain cells [1,2,3]
We examined the cell morphology of the BV2 microglial cells that the LPS-treated cells was (500 ramified compared the control group, weremicroglial incubated with scoparone μM) in the presence orto absence of LPS (200 ng/mL).indicating
The activation of the microglia resulted in the elevated levels of certain neurotoxic and activation of the microglial cells can be effectively prevented by bioactive compounds that have proinflammatory mediators, which can result in the severe damage of brain cells, and in turn lead been isolated from natural resources [4,5,24]
Summary
Neurons and microglial cells are the integral part of the central nervous system, in which the microglial cells occupy 5%–10% of the brain cells [1,2,3]. Scoparone (6,7-dimethoxycoumarin) is one of the major active natural bioactive compounds in chestnut inner shell (Castanea crenata), which has multiple beneficial activities including anticancer, LPS-stimulated TRIF-dependent signaling induces the transcription factor, interferon regulatory factor 3. Scoparone has the neurite-outgrowth potential through its neuroinflammation in the microglial cells has not been reported, the focus of the present stimulation of the upstream steps of ERK, cyclic ACP-dependent protein kinase, protein-kinase C, and study is the evaluation of scoparone’s effectsbyand its molecular. The protective role of scoparone against neuroinflammation in the microglial cells has not been reported, the focus of the present study is the evaluation of scoparone’s anti-neuroinflammatory effects and its molecular mechanism in LPS-induced BV-2 microglial cells. Effect of Scoparone on LPS-Induced Cellular Viability and Nitrite Production in BV-2 Microglial Cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
