Abstract
Čerenkov radiation produced by 32P makes possible a convenient and efficient method for determination of 32P in virtually any sample utilizing conventional scintillation counters. Organic solvents provide high absolute efficiencies—the highest efficiency measured, approximately 80%, was obtained with toluene. Approximately 50% absolute efficiency is obtained in aqueous solutions with very little variation when strong acids, bases, or salts are present. Paper and silica gel give similar efficiencies with no liquid present because Čerenkov radiation occurs in polyethylene and in glass. Samples are completely unaltered by the counting. Counting conditions are constant for all samples. The efficiency varies with volume, solution composition, and vial material. Efficiencies relative to water for a wide variety of aqueous solutions and organic solvents have been determined using a rapid, highly accurate method.
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