Abstract
To determine the role of remote perconditioning (RPeC) on renal function and histology in an animal model of unilateral renal ischemia and reperfusion (IR) injury. Rats were subjected to 60min unilateral renal ischemia. RPeC protocol was the application of four cycles of 5min IR of left femoral artery during renal ischemia. Assessments of histological changes and renal function were made 24h, 1week, or 3weeks later. 99mTc-DMSA scan was performed using a small-animals SPECT system. 24-h reperfusion decreased the 99mTc-DMSA uptake in the left kidney compared to the intact kidney of control animals. RPeC group has higher uptake compared to the IR group. After 1week and 3weeks, uptakes were gradually increased in both groups and no differences were observed. Severe morphological changes in the ischemic kidneys of both groups were observed after 24h which attenuated after 1week and 3weeks. Moreover, no differences in creatinine and BUN levels between IR-treated and intact animals were observed. These data suggest that RPeC exerts a partially transient improvement in the renal function in the first day after reperfusion. However, long-term follow-up study showed no beneficial effects of RPeC. Moreover, noninvasive 99mTc-DMSA scan revealed a suitable tool in the follow-up evaluation of recovery process in the unilateral renal IR injury models.
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