Abstract
The human dermis presents an ongoing problem for regenerative medicine. Current medical management uses various acellular dermal matrices on wound sites. The challenge for scientists is to examine, then to question accepted conventional wisdom and to present new concepts. In this paper, Autologous Cell Therapy will be described by using cell culture of autologous dermal fibroblasts and their extracellular matrix as a foundation for rebuilding the dermis in conditioned wound beds. This proposal seems to create a conflict with the medical approach to keeping a wound bed “moist”.
Highlights
While clinicians dictate a mode of wound care treatment, a scientist seeks an understanding of cellular processes occurring under the designated applied dressing
An initial “moist” wound bed followed by an air-“dry” wound bed to enable the resident dermal fibroblasts to fully go to work
Biotechnology companies have not made use of the natural cell secretions, which include growth factors and cytokines to be found in conditioned medium (CM) of dermal fibroblast cell cultures derived from healthy subjects
Summary
While clinicians dictate a mode of wound care treatment, a scientist seeks an understanding of cellular processes occurring under the designated applied dressing. Pressing a finger into a former wound site reveals a depression which can be not immediately apparent at first sight This author has recently described the signature side-on packing configuration at confluence of human dermal reticular fibroblasts at an angled vertical in vitro, obtained by non-enzymatic isolation, using 20% foetal bovine serum (FBS) [11]. Expensive laboratory-created cultured epithelial autografts (only one major type of epidermal cell in these CEAs: the keratinocyte) were used a burns treatment without regard for the underlying dermis and its varied cellular content This haste to medically cover up a wound or burn has been described by Ovington 2007 [13] as “cover and conceal”. An initial “moist” wound bed followed by an air-“dry” wound bed (once the granulation tissue has taken hold) to enable the resident dermal fibroblasts to fully go to work
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