SCIENTIFIC SUBSTANTIATION OF THE PERMISSIBLE DAILY DOSE OF A TECHNICAL PRODUCT DERIVED FROM CHLOROACETAMIDES

Abstract

Introduction. The scientific basis for the safe use of pesticides are comprehensive sanitary and toxicological studies to study the parameters of their toxicity and biological action, hygienic regulation for the purpose of scientific justification of regulations and safety measures when working with them. Purpose of research. The study of parameters of acute and chronic toxicity, biological actions technical product derived chloracetamide, at oral intake of mammals (rats), the rationale of the acceptable daily intake (ADI) for humans. Material and methods of research. In acute experiments used white male rats weighing 210-220 g Tested doses 1000 and 4000 mg/kg of body weight. Statistical group for each dose consisted of 6 animals. Chronic experiment was carried out on male rats with body weight 180-190 g. Doses were tested: 3,5; 17,6 and 70,0 mg/kg body weight. Statistical groups of each dose and control group included 20 animals. In the dynamics of the experiment, the state and behavior of animals, water and food consumption were observed, the terms of death were fixed, changes in body weight, physiological, biochemical and hematological parameters were recorded. The value of the permissible daily dose was determined by the ratio of the maximum inactive dose to the reserve ratio. Results. As a result of the research it was found that LD50 of the studied compound is 2172 ± 370 mg/kg. In the chronic experiment, the dose of 3.5 mg/kg b.w. does not cause significant changes in all the studied parameters, at a dose of 17.6 mg/kg b.w. single changes, the dose of 70.0 mg/kg. had a polytropic effect on the body of experimental animals. Discussion. The studied technical product for acute oral toxicity refers to low-hazard compounds. It was found that multiple oral intake of the studied product at a dose of 70.0 mg/kg weight body revealed changes in the state of the Central nervous system, and the analysis of biochemical and hematological parameters of blood showed that changes in carbohydrate, lipid and lipoprotein metabolism, aminobelic metabolism occur in animals. Conclusions. The studied compound for acute oral toxicity according to the hygienic classification of pesticides (SanPiN 1.2.2584-10) belongs to the 4th hazard class. Dose: 70,0 mg/kg weight body - acting; 17,6 mg/kg weight body - threshold; 3.5 mg/kg weight body - inactive. The permissible daily dose (DSD) for a person is 0.035 mg/kg.