Abstract
Scientific CCD cameras with high sensitivity have been widely used for low-light-level quantitative fluorescence microscopy (QFM) in cell biology. These state-of-the-art scientific CCDs offer several advantages over others, but what's new is not always the best choice for every application, and their specifications don't always tell the whole story. So, many pivotal technologies are poorly elucidated which would cause serious errors in QFM. The present study systematically analyzes theoretical reasons and technical sources of discrepancies occurring in QFM. In order to avoid the errors and achieve the accurate determination, this study proposes solutions and suggests some critical measures in both theoretical and technical aspects. Therefore, this analysis can be a valuable tool in scrutinizing multifarious parameters and clarifying proper usages of a scientific CCD camera, especially in low-light-level quantitative fluorescence imaging measurement.
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