Sciatic nerve protein composition in normal and dystrophic C57BL/6J mice.

Abstract

Proteins were extracted from homogenized sciatic nerves of normal C57BL/6J and dystrophic C57BL/6J dy2J/dy2J mice and were separated on SDS-polyacrylamide slab gel electrophoresis. Proteins visualized on Coomassie blue-stained gels were resolved into 43 bands. These were quantitated by densitometric scanning and continuous plotting of OD 595, then heights and areas of individual peaks were measured. The relative proportions of 5 proteins of intermediate molecular weight (37-92 kDa) were greater in dystrophic than normal nerves. These augmented proteins were not related to myelin or nuclear histone proteins.