Abstract
At present, just a few of the available in vitro genotoxicity tests measure DNA strand breaks and their repair in cells. The comet assay as the considered “gold standard” is still not standardized and does not allow full automation so far. In contrast, most steps of the fluorimetic analysis of alkaline DNA unwinding (FADU) assay could be automated which allows precise dispensing of solutions and control of temperature and process time during the assay. This progress has led to improved reproducibility and throughput, easier handling, and enhanced sensitivity and speed.
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