Abstract
Background:Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in females worldwide. Schleichera oleosa (kusum tree) belongs to the Sapindaceae family commonly found in many states of India. This plant is traditionally being used in various pathological conditions. Methods: In vitro studies were performed using seed extract of Schleichera oleosa. Different concentrations of seed extracts were treated on MCF-7 breast cancer cell line and its effect on migration and colony formation were observed. BRCA1 and p16 gene expression was analyzed by real-time PCR and Western blotting. Results:We have analyzed anticancer and anti-metastatic effects of seed extract in breast cancer and IC50 was 140µg/ml concentration. Further, its inhibitory role in cell migration and colony formation was at 140µg/ml (P<0.0001) concentration and reduced significantly growth of sphere at 140 µg (P<0.0031) and 150µg (P<0.0010) concentration after 5 days of treatment. The apoptosis study was shown a significant increase at 140 µg (P<0.0001) in apoptotic cells. Expression of BRCA1 and p16 were found to be over-expressed as 1.4 and 1.7 fold, respectively, at 140µg/ml concentration after 24 h of treatment at the transcription level. BRCA1 protein was up-regulated but p16 expression down-regulated at 140 to 150µg/ml (One-Way ANOVA, P<0.0001) concentration. Conclusion:In this study, we found a significant role of S. Oleosa seed extract has an anti-cancer as well as anti-metastatic via up-regulation of BRCA1 and p16 genes in breast cancer cells.
Highlights
Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in females worldwide (Ferlay et al, 2019)
Oleosa on the basis of IC50 in MCF-7 cells and phytochemical constituents identified by GC-MS, which induces apoptosis in endothelial cells reported earlier (Palanuvejet al., 2008; Li et al, 2017).We have identified its anti-cancer as well as anti-metastatic role in breast cancer by up regulation of BRCA1 and p16 genes
Inhibitory concentration IC50 calculation of plant extract The MTT assay was performed in MCF-7 cells using plant extracts
Summary
Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in females worldwide (Ferlay et al, 2019). Schleichera oleosa (kusum tree) belongs to the Sapindaceae family occurs at the foothills of the Himalayas and commonly found in many states of India such as South India, Chhattisgarh, Bihar, West Bengal, Madhya Pradesh and Uttar Pradesh. Schleichera oleosa (kusum tree) belongs to the Sapindaceae family commonly found in many states of India This plant is traditionally being used in various pathological conditions. Different concentrations of seed extracts were treated on MCF-7 breast cancer cell line and its effect on migration and colony formation were observed. Oleosa seed extract has an anti-cancer as well as anti-metastatic via up-regulation of BRCA1 and p16 genes in breast cancer cells
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