Schistosoma mansoni: in vitro uptake and incorporation of glycine by adult worms

Abstract

1. 1. The uptake of [ 3H]glycine into an ethanol-extractable intracellular pool and its simultaneous incorporation into protein (TCA insoluble fraction) have been measured in adult male and female Schistosoma mansoni incubated, in vitro, in complex media. 2. 2. The patterns of uptake and incorporation were similar in male and female schistosomes incubated in media containing serum, and also in male worms incubated in the presence or absence of serum. 3. 3. The amounts of [ 3H]glycine uptake and incorporation were directly proportional to the extracellular glycine concentration and were identical in male worms (in the presence or absence of serum) and female worms. 4. 4. Emetine at a concentration producing a 95% inhibition of protein synthesis was without effect on the uptake of [ 3H]glycine into the ethanol-extractable pool in male worms.