Schistosoma japonicum Soluble Egg Antigens Attenuate Invasion in a First Trimester Human Placental Trophoblast Model

Emily A Mcdonald,Surendra Sharma,Sunthorn Pond-Tor,Jonathan D Kurtis,Luz Acosta,Eric S White,Jennifer F Friedman,Ling Cheng,William Evan Secor

doi:10.1371/journal.pntd.0002253

Abstract

BackgroundSchistosomiasis affects nearly 40 million women of reproductive age, and is known to elicit a pro-inflammatory signature in the placenta. We have previously shown that antigens from schistosome eggs can elicit pro-inflammatory cytokine production from trophoblast cells specifically; however, the influence of these antigens on other characteristics of trophoblast function, particularly as it pertains to placentation in early gestation, is unknown. We therefore sought to determine the impact of schistosome antigens on key characteristics of first trimester trophoblast cells, including migration and invasion.MethodsFirst trimester HTR8/SVneo trophoblast cells were co-cultured with plasma from pregnant women with and without schistosomiasis or schistosome soluble egg antigens (SEA) and measured cytokine, cellular migration, and invasion responses.ResultsExposure of HTR8 cells to SEA resulted in a pro-inflammatory, anti-invasive signature, characterized by increased pro-inflammatory cytokines (IL-6, IL-8, MCP-1) and TIMP-1. Additionally, these cells displayed 62% decreased migration and 2.7-fold decreased invasion in vitro after treatment with SEA. These results are supported by increased IL-6 and IL-8 in the culture media of HTR8 cells exposed to plasma from Schistosoma japonica infected pregnant women.ConclusionsSoluble egg antigens found in circulation during schistosome infection increase pro-inflammatory cytokine production and inhibit the mobility and invasive characteristics of the first trimester HTR8/SVneo trophoblast cell line. This is the first study to assess the impact of schistosome soluble egg antigens on the behavior of an extravillous trophoblast model and suggests that schistosomiasis in the pre-pregnancy period may adversely impact placentation and the subsequent health of the mother and newborn.

Highlights

Schistosomes are parasitic worms endemic to many parts of Africa, South America and Southeast Asia
Soluble egg antigens found in circulation during schistosome infection increase pro-inflammatory cytokine production and inhibit the mobility and invasive characteristics of the first trimester HTR8/SVneo trophoblast cell line
This is the first study to assess the impact of schistosome soluble egg antigens on the behavior of an extravillous trophoblast model and suggests that schistosomiasis in the pre-pregnancy period may adversely impact placentation and the subsequent health of the mother and newborn

Summary

Introduction

Schistosomes are parasitic worms endemic to many parts of Africa, South America and Southeast Asia. They represent a significant disease burden in endemic regions, and have been estimated to be responsible for as many as 13–15 million disabilityadjusted life years (DALYs) lost per year, with the true number potentially much higher [1]. A 2002 World Health Organization policy statement recommended the use of praziquantel in pregnant and lactating women [4], many women still experience multiple cycles of pregnancy and lactation with schistosomiasis. We sought to determine the impact of schistosome antigens on key characteristics of first trimester trophoblast cells, including migration and invasion

Methods

Results

Conclusion