Schisandrin B Suppressed the Proliferation of MM Cell Lines Via IL-6

Abstract

Background: Multiple myeloma (MM) is a hematologic malignancy. Schizandrin B (Sch B) is one of the main dibenzocyclooctadiene lignans which is found in schisandra chinensis. Sch B has been previously demonstrated to exhibit antitumor properties. However, the effect of Sch B on MM remains nuclear. Materials and Methods: The impact of Sch B on the proliferation of MM cell lines was determined by CCK-8 assay. The cell cycle and apoptosis of MM cell lines were evaluated by flow cytometry. RT-PCR to identify the effect of Sch B on the chemokines CCL2, CCL3 and CCL14 in MM cell lines. IL-6 and VEGF concentrations of serum and cell-free supernatants were tested by ELISA. Result: Our studies revealed that Sch B suppressed the proliferation of MM cell lines via IL-6. Sch B induced cell cycle of MM cell lines arrest at G0/G1 phase and increased its apoptosis. Sch B suppressed the mRNA expression of the chemokines CCL2, CCL3and CCL14 in MM cell lines. Conclusion: Sch B suppressed the proliferation of MM cell lines via IL-6. These findings suggest that Sch B may be a promising traditional Chinese medicine for MM.