Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis

Xueni Wang,Yuzhou Pang,Yimin Zhang,Linyao Wei,Xiuling Liao

doi:10.1038/s41598-020-71564-z

Xueni Wang, Yuzhou Pang + Show 3 more

Open Access

https://doi.org/10.1038/s41598-020-71564-z

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Abstract

Schisandrin B (SchB) is the highest content of biphenyl cyclooctene lignans in Schisandra chinensis. It has been reported to have a variety of pharmacological effects, including anti-inflammatory, anti-oxidant, anti-cancer, heart protection, liver protection. In this study, we found that SchB can promote the proliferation of MC3T3-E1 subclone 14 cells. Meanwhile, we found that SchB can regulate the BMP2-SMADs signaling pathway by increasing gene and protein expression of those relative biomolecules. Furthermore, SchB can raise the RUNX2 and SP7 expression in both mRNA and protein levels. Since the role of BMP2-SMADs-RUNX2-SP7 signaling axis in osteoblast proliferation and differentiation has been well documented. The present experimental findings indicate that SchB could promote the proliferation and differentiation of osteoblasts through BMP2-SMADs-RUNX2-SP7 signaling axis.

Highlights

Schisandrin B (SchB) is one of the lignans with high content from Schisandra chinensis[1]
Osteoblast proliferation plays an important role in bone maintenance and development
We found that SchB can promote the proliferation of MC3T3-E1 subclone 14 cells and up-regulate the gene and protein expression of biomolecules in BMP2-SMADS signaling pathway

Summary

Introduction

Schisandrin B (SchB) is one of the lignans with high content from Schisandra chinensis[1]. It is worth noting that SchB has been reported to ameliorate chondrocytes inflammation and osteoarthritis by inhibiting NF-κB and MAPK signaling p athways[6]. These findings suggest that SchB may play a potential role in alleviate bone disease. MC3T3-E1 subclone 14 cells are often used to study the proliferation and differentiation of osteoblasts[7,8,9] It is well known BMP2-SMADs signaling pathway play a key role in mediating osteoblast differentiation and osteogenesis[10]. The effects of SchB on the proliferation of MC3T3-E1 subclone 14 cells were evaluated by MTT assay, and the effects of SchB on the expression of genes and proteins related to BMP2-SMADs-RUNX2-SP7 signaling axis were investigated by quantitative PCR and western blot, respectively

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